The present invention discloses a nanoparticle nucleic acid carrier containing hyperbranched polylysine (HBPL) and its application, and relates to the field of biomaterials. The nucleic acid carrier is capable of forming stable nanoparticles by electrostatic binding with nucleic acid substances, and is conveniently accessible and reproducible in terms of both preparation method and means of application. The nanoparticle nucleic acid carrier containing hyperbranched polylysine provided by the present invention has low cytotoxicity, and can successfully load nucleic acid substances into cells to obtain a high transfection rate, and compared with the best existing gene transfection carrier polyethylenimine (PEI), the nucleic acid carrier in the present application can exhibit a higher transfection rate and lower cytotoxicity, and has good It has good application prospects in gene therapy, nucleic acid vaccine and other fields.
